ANTIMICROBIAL METABOLITE OF ZINGIBERACEAE ESSENTIAL OILS USING RESAZURIN A RAPID COLORIMETRIC DETECTION

Abstract

An antimicrobial assay using broth microdilution is the method accepted and approved by Clinical and Laboratory Standards Institute (CLSI). This technique was in vitro and minimized a volume of the medium that was performed the susceptibility of antimicrobial activity using resazurin rapid colorimetric detection. The technique had performance testing antimicrobial activities of essential oil from Zingiberaceae; Curcuma longa L, C. zedoaria (Christm.) Roscoe, C. aromatica Salisb, C. comosa Roxb., and Zingiber cassumunar Roxb. An approval this technique was confirmed using agar disc diffusion assay. All the essential extracted had bioactives against all testing pathogenic gram positive and gram negative bacteria and infectious yeast Candida albicans especially C. aromatica and C. comosa had inhibited Pseudomonas aeruginosa more than Ampicillin, antibacterial drug. Hence, it is expected that, from these results, C. aromatica and C. comosa rhizomes from different habitats should be used more rationally and effectively as natural pharmaceutical, culinary and cosmetic additives in the future.